植物与病原菌互作中活性氧的检测方法

Methods for assay of active oxygen species on plant-pathogen interaction

活性氧迸发是植物对病原物应答的最早期反应之一.活性氧与植物抗病性密切相关.活性氧的检测方法主要有:分光光度法、荧光法、化学发光法、组织化学法、金属沉淀法等.对活性氧的测定是对其功能以及与其它信号分子间关系研究的重要手段.

Oxidative burst is one of the earliest responses in plant resistance to pathogen attacks. The rapid transient production of active oxygen species plays an important role in plants＇ defense strategy. The method of assay for generation of active oxygen species includes spectrophotometry, fluororesence, chemiluminescence, histochemistry, etc. The detection of active oxygen species is in favor of investigating its function and the connection with other signals.