脉冲电场对细胞杀伤效果及其原理的研究

Influence of different pulsed electric fields on HL-60 cell death

使用两种不同上升沿的脉冲电场(1μs和100ns)电击悬浮HL-60细胞,观察脉冲强度、数目、电击后恢复时间等参数的变化对细胞死亡率和细胞形态的影响。实验表明,脉冲强度、数目的增加导致死亡率也增加。电击后,100ns脉冲作用的细胞的死亡率比1μs的增加得更快。本文还进一步讨论不同上升沿电场在作用机理上的差异。

Pulsed electric field with different ramp slopes were used to irradiate the suspended HL-60 cells. The experiment settings are designed to produce pulsed electric field on sample with the intensity peak of 3.82MV/m within lOOns and 1μs, respectively. Cell death rates are assessed after electric irradiate according to the di＇fferent field intensity, pulse number and recovery time between samples. Cell death rate increases for both ramp slopes when the field intensity or pulse number increases. Cell death increases faster after irradiated by lOOns pulsed electric field than that of 1μs. Cell death rates are recorded after stimulation with recovery time interval of 30 minutes. Cell death rate of lOOns pulsed electric field rises slowly and reaches its peak later than that of 1μs pulsed pulsed electric field causes the death of cell in a more indirectly way contrasting with electric field. It means the lOOns the 1μs pulsed electric field