摘要
用循环伏安法制备了聚L-精氨酸修饰玻碳电极,并研究了抗坏血酸在修饰电极上的电化学行为,建立了测定抗坏血酸的新方法.在pH7.5的磷酸盐缓冲溶液中,抗坏血酸在修饰电极上产生一氧化峰,峰电位为0.050V(对AgA/gCl电极).用线性扫描伏安法测定抗坏血酸的线性范围为5.0×10-6~5.0×10-3molL/,检出限为2.0×10-6molL/.用于药物中抗坏血酸的测定,结果满意。
A poly(L-arginine) chemical modified electrode(PLA/GCE) was fabricated by electrochemical immobilization of L-arginine on a glassy carbon electrode(GCE) through cyclic voltammetry(CV) The voltammetric behavior of ascorbic acid and a cyclic vohammetric method for the determination of ascorbic acid have been studied at the poly(L-arginine) modified electrode. In pH 7.5 phosphate buffer solution, the modified electrode gave a oxide peak at 0. 050 V (vs. Ag/AgC1). The linear response was obtained in the range of 5.0 ×10^6 - 5.0 ×10^-3 mol/L. The detection limit was 2. 0 ×10^-6 mol/L. The method has been successfully applied to the determination of ascorbic acid in drug with satisfactory results.
出处
《淮北煤炭师范学院学报(自然科学版)》
2007年第1期24-27,共4页
Journal of Huaibei Coal Industry Teachers College(Natural Science edition)
基金
安徽省教育厅自然科学基金(2004KJ320
2005KJ140)
安徽省绿色化学重点实验室项目基金K(LSF(I)07)
淮北市科技计划基金资助项目(040145)