玉米蛋白粉水解多肽对光磷水蜈蚣的生根抑制活性

Root-inhibiting Activity of Hydrolized Peptides of Corn Gluten Meal on Smoothscale Kyllinga(Kyllinga brevitolia var.leiolepis)

以木瓜蛋白酶、中性蛋白酶为水解酶,以玉米蛋白粉为底物,制备出不同酶解时间条件下的玉米蛋白水解多肽。用培养皿生物分析法检测不同玉米蛋白水解多肽在不同浓度(0.5 mg/mL、1 mg/mL、2 mg/mL、5 mg/mL)条件下对光磷水蜈蚣(Kyllinga brevitoliavar.leiolepis)种子生根抑制活性。结果表明:①两种蛋白酶不同水解时间制备的玉米蛋白粉水解多肽对光鳞水蜈蚣种子萌发生根均有抑制作用,且随着浓度增高生根抑制活性增强;②木瓜蛋白酶在水解3 h和6 h制得玉米多肽的生根抑制活性明显优于水解9 h和12 h制得的玉米多肽,而中性蛋白酶在不同水解时间水解玉米蛋白粉所得的水解多肽对光磷水蜈蚣的抑制活性无显著性差异;③木瓜蛋白酶制备的玉米多肽比中性蛋白酶制备的玉米多肽生根抑制活性强,并达到极显著性差异水平(P<0.01)。

Corn gluten peptides were prepared by two proteases in different hydrolysis times and their root-inhibiting activity on Kyllinga brevitolia vat. leiolepis was conducted in petri dish bioassays at rate of 0, 0.5 mg/mL,1 mg/mL,2 mg/mL, 5 mg/mL. The result showed： （1）Corn gluten peptides prepared by two proteases have root-inhibiting activity on Kyllinga brevitolia vat. leiolepis, and their bioactivity becomes greater with the increasing concentrations. （2） Inhibitory activities of peptides changed with hydrolyzing time using papain to hydrolyze CGM and corn gluten peptides prepared at hydrolysis time of 3 hours and 6 hours exhibited more inhibitory activity than that of 9h and 12h. On the contrary, no difference was found for the peptides prepared by neutrase in different hydrolysis time. （3） Compared with papain, peptides prepared by neutrase is less inhibitory active and the ANOVA analysis shows highly significantly difference. Therefore, as a hydrolysase, papain can be taken priority to prepare the corn gluten peptides.