甲胺磷降解菌响应曲面法优化研究

Methamidophos-degrading Bacteria for the Extracellular Polysaccharide Fermented by Using Response Surface Methodology

从受甲胺磷污染的土壤中分离筛选出甲胺磷降解菌,采用响应曲面法对影响降解菌的5个因素培养温度(X1)、pH值(X2)、甲胺磷的浓度(X3)、装液量(X4)、接种量(X5)的最佳水平范围进行了研究。通过对二次多项回归方程求解得知,在上述自变量取值分别为:温度28℃,pH值7.0,甲胺磷浓度1 500 mg/L,装液量100 mL,接种量10%时,甲胺磷降解率为最大,预测值为73.65%。

One of methamidophos-degrading bacteria was isolated from polluted soils. Response surface methodology（RSM） based on a five-level five-factor central composite rotatable design of experiments was used to optimize the optimal levels of five important factors,namely temperature（X1 ）,pH value（X2 ）, concentration of methamidophos （X3）, the medium volume （X4）, the quantity of inoculum （X5）. Using the quadratic regression model, the optimum concentrations were determined： temperature 28℃, pH value 7.0, concentration 1 500 mg/L, medium volume 100 mL and inoculum amount 10% ,respectively, the predicted yield of the methamidophos degrading rate was 73.65%.