代谢表面活性剂菌处理含油污泥的研究

Study on treatment of oily sludge by biosurfactant-metabolized microbes

试验采用异位生物修复技术堆肥法,对某炼厂油泥进行生物修复处理研究。用微生物代谢的表面活性剂对油泥进行预处理,洗脱油泥中部分油分后进行堆肥试验,投加从油田含油土壤中获得的以石油为唯一碳源、代谢高效生物表面活性剂的微生物C-2菌、F-2菌以及无机营养物和疏松剂(锯末),降解油泥中的石油污染物。经过外源微生物和内源微生物共同作用120d,油泥中的石油烃总量由22910mg/kg下降到3000mg/kg以下。试验利用色谱-质谱联用方法分析了降解前后石油组分的变化。菌株经传统方法鉴定为蜡状芽孢杆菌、枯草芽孢杆菌。

The petroleum refinery＇s oily sludge was bioremediated by composting with native flora and selected microorganisms which can metabolize bio-surfactants. At first, the oily sludge was pretreated by the bio-surfactants to decrease the total petroleum hydrocarbon （TPH）, then the pretreated sludge was biodegradated by composting with selected strains named C-2, F-2 which can use oil as sole carbon source and metabolize good surfactants. With the selected strains, bulk agent （sawdust）, inorganic nutrition and water were provided, the TPH of oily sludge reduced from 22 910 mg/kg to less than 3 000 mg/kg after 120 days. The strains are identified as Bacillus cereus and Bacillus subtilis by traditional method. The variety of oil is analyzed by GC/MS before and after biodegradation.