姜黄素对动脉粥样硬化家兔核转录因子-κB的影响

Influence of curcumin(姜黄素) on the expression of nuclear factor-κB in the aorta of rabbits with atherosclerosis

目的:探讨姜黄素抗动脉粥样硬化(AS)的部分机制。方法:给30只雄性日本大耳白家兔高脂饲料喂食2个月,将成功复制AS模型的20只家兔随机分成两组,各10只。基础饲料喂养的同时,姜黄素治疗组每日给予姜黄素200 mg/kg灌胃,对照组每日给予等量生理盐水灌胃,共治疗1个月。观察两组治疗前后血浆甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)的变化。治疗后处死家兔,观察两组主动脉内膜脂质斑块面积比。取主动脉弓做病理切片,用免疫组化法检测主动脉壁核转录因子-κB(NF-κB)阳性表达。结果:结束时两组各有8只家兔进入统计学分析。治疗前两组家兔呈典型的高脂血症。治疗1个月后,姜黄素治疗组TG、TC和LDL-C均较对照组显著降低,HDL-C较对照组显著升高,其中TG降低了23.5%(P=0.024),TC降低了21.6%(P=0.020),LDL-C降低了30.3%(P=0.009),HDL-C升高了16.3%(P=0.040);姜黄素治疗组主动脉内膜脂质斑块面积比低于对照组(P=0.025);免疫组化显示,姜黄素治疗组NF-κB阳性细胞表达率明显低于对照组(P=0.005)。结论:姜黄素可以显著降低AS家兔主动脉壁NF-κB的表达,抑制AS的形成。

Objective： To investigate the effect of curcumin （姜黄素） on the expression of nuclear factor -κB （NF-κB） in the aorta of rabbits with atherosclerosis （AS）. Methods： Thirty male Japanese white rabbits were fed with a high - cholesterol diet for 2 consecutive months. Finally, 20 rabbits of AS model were established. They were randomly divided into two groups： the control group and treatment group （each n=10）. Both groups were fed with a standard diet for next 1 month. Additionally, the treatment group received an oral curcumin at a dose of 200 mg/kg body weight, and the control group received an oral curcumin- free equal volume of normal saline solution daily during the experiment. Before and after the curcumin therapeutic period, the blood samples were collected in both groups. Plasma was separated and used for the assessment of total cholesterol （TC）, low density lipoprotein -cholesterol （LDL -C）, high density lipoprotein -cholesterol （HDL- C） and triglyceride （TG）. After treatment, all animals were killed. Entire aortas were rapidly dissected out, and the ratio of lipid plaque area was examined. The aortic arch was selected for histological examinations, and the positive expression of NF-κB in the aorta was examined with semiquantitative analysis of immunohistochemistry. Results ： Finally, each of the two groups had eight rabbits in the analysis. The animals fed with high -cholesterol diet had resulted in marked hypercholesterolemia. After treatment for 1 month, compared with the control group, the TG in the treatment group was decreased by 23.5% （P=0.024）, TC by 21.6% （P=0.020） and LDL-C by 30. 3% （P=0.009）, and HDL-C was significantly increased by 16.3% （P= 0. 040）. The ratio of lipid plaque area reduced obviously in treatment group as compared to that of control group （P = 0.025 ）. The rate of positive cell of NF -κB immunohistochemistry expression was signifieantly lower in treatment group than that in eontrol group （P=0. 005）. Conclusion： Curcumin can decrease the expression of NF -κB in the aorta and attenuate AS development in rabbits.