黄芩甙和奥曲肽对重症急性胰腺炎大鼠心脏损伤保护作用的比较

Study of protecting effects of baicalin(黄芩甙) and octreotide on heart injury in rats with severe acute pancreatitis

目的:比较黄芩甙和奥曲肽对重症急性胰腺炎(SAP)大鼠心脏损伤的保护作用及其机制。方法:采用改良Aho法制备SAP大鼠模型。术后3、6和12 h腹主动脉取血,观察各组大鼠血中淀粉酶、内毒素、一氧化氮(NO)、超氧化物歧化酶(SOD)、丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)、磷脂酶A2(PLA2)、内皮素-1(ET-1)变化并计算大鼠死亡率,应用组织微阵列技术制备心肌组织切片观察心肌组织病理变化和心肌组织核转录因子-κB(NF-κB)、Bax、Bcl-2蛋白的表达。结果:1假手术组全部存活;模型组12 h死亡率为33.33%;治疗组为0。2两个治疗组血中淀粉酶、内毒素、NO、MDA、TNF-α、PLA2、ET-1含量均较模型组降低,SOD活性升高;其中黄芩甙组内毒素、PLA2和MDA含量下低较明显,奥曲肽组TNF-α和ET-1含量降低较为明显(P<0.05或P<0.01)。3 h和6 h模型组心肌组织Bax蛋白有表达,6 h奥曲肽和黄芩甙组心肌组织Bcl-2蛋白有表达;除奥曲肽组6 h有1只大鼠出现心肌细胞凋亡(凋亡率20%)外,其余均为阴性;不同时间点各组NF-κB蛋白表达均为阴性。3两个治疗组心肌组织病理改变均较模型组减轻,且奥曲肽组疗效更好。结论:黄芩甙和奥曲肽对SAP大鼠心脏损伤具有保护作用,其机制与抑制炎症介质有关,但两药疗效与凋亡间的关系不明确。组织微阵列技术用于组织病理学研究效率高、成本低、代表性好。

Objective： To compare the protecting effects and mechanisms of baicalin （黄芩甙） and octreotide on heart injury in rats with severe acute pancreatitis （SAP）. Methods： SAP model was replicated by improved Aho method. The contents of amylase, endotoxin, nitric oxide （NO）, superoxide dismutase （SOD）, malondialdehyde （MDA）, tumor necrosis factor-a （TNF-α）, phospholipase A2（PLA2） and endothelin- 1 （ET- 1） were determined at 3, 6 and 12 hours after operation, and the mortality was calculated. The technique of tissue microarrays in pathological examination was Used to prepare myocardial tissue section, and thechanges of pathology and myocardial nuclear factor -κB （NF -κB）, Bax, Bcl - 2 protein expression levels were observed. Results： ①All animals in sham operation group were survived. The mortality of model group was 33.33 %, while were both 0 in treatment groups at 12 hours. ②The contents of amylase, endotoxin, NO, MDA, PLA2, ET - 1 and TNF -α of two treatment groups were lower, while the SOD activity was higher than those of the model group at different time points. The endotoxin, PLA2 and MDA in baicalin treatment group, while TNF-α and ET - 1 in octreotide treatment group were more significantly decreased （P〈0.05 or P〈0. 01）. The Bax protein expressions of myocardial tissues were observed at 3 and 6 hours in model group. The Bcl - 2 protein expressions of myocardial tissues were observed at 6 hours in two treatment groups. There was only one rat with myocardial cell apoptosis in octreotide treatment group at 6 hours （apoptosis rate 20% ）, and no apoptosis was seen in the other groups. The NF -κB protein expression was negative at all time points in all groups. ③The pathological myocardial changes were milder in treatment groups than those in model group, and the therapeutic effects were better in octreotide treatment group. Conclusion： Baicalin and octreotide have protecting effects on heart injury of rats with SAP, and the mechanisms are related to their inhibition of inflammatory mediators. The relation between their therapeutic effects and apoptosis is unknown. The advantages of tissue microarrays in pathological examination include energy saving, highly efficient and representative.