含氯消毒剂对微宇宙中细菌呼吸作用的影响

Effect on the respiration of bactria in microcosm by the disinfectant of chlorine

目的评价含氯消毒剂(次氯酸钠)对微宇宙中细菌呼吸量的影响。方法采用景观水及其底泥为研究对象,人工构建微宇宙,连续投加消毒剂并对细菌呼吸量和细菌总数进行动态监测。结果2.5mg/L以下剂量组消毒剂在消毒初期对细菌有一定的刺激作用,呼吸量增加,持续消毒后,主要体现在对细菌的杀灭和抑制上,整体呼吸下降;而高剂量组(20mg/L)消毒剂初始即表现出对细菌较强的抑制和杀灭,造成呼吸量迅速下降。停止投加消毒剂后,细菌呼吸量可以逐渐恢复到投加前水平,这种变化与该微生物生态环境中细菌总数的变化基本一致。结论微宇宙中细菌呼吸量的变化与消毒剂的投加量及持续时间有关,10mg/L以下低剂量组对微宇宙中微生物代谢活性影响较小。

Objective To observe respiratory volume of bacteria as the physiology activity index to evaluate the effect of sodium hypochlorite （NaClO） on the microenvironment. Methods The water and soil from Wuban Muoshui Lake were selected as research object. Man-made microcosms were designed and constructed. The sodium hypochlorite was put into the microcosms every 24 hour for 13 days. The bacteria respiratory volume and the general bacterial population were observed. Results The results showed that the bacteria in the low-dose disinfectant were stimulated and its respiration volumes were increased in the beginning of the experiment. But several days later, the bacteria were inhibited or killed predominantly which led to the decrease of its respiration volumes. In high-dose group, the bacteria were killed obviously in the beginning and their respiration volumes decreased immediately. After the disinfectant was given up, the respiratory volume resumed gradually to the initial condition. This change process accorded with the general bacterial population as a whole. The respiratory volume of bacteria was related with the dose of disinfectant. Conclusion The change of the respiratory volume of bacteria was related with the dose of sodium hypochlorite. The disinfectant effect on the metabolic activity of microorganism would be lighter if it under the dose 10mg/L.