热休克蛋白70-1基因多态性与缺血性脑卒中易感性的前期研究

Study of genetic polymorphism of heat shock protein 70-1 associated with susceptibility to ischemic stroke

目的探讨热休克蛋白70-1(HSP70-1)基因多态性与缺血性脑卒中的关系。方法采用1∶1配比病例-对照研究设计,选择深圳市两家综合性医院的116例缺血性脑卒中患者为病例组,同时选择年龄、性别匹配116例健康者作为对照组。采用聚合酶链反应-限制性片断长度多态性(PCR-RFLP)方法检测HSP70-1多态性,同时按照流行病学方法设计调查表进行问卷调查。结果HSP70-1基因型及等位基因频率在两组中的分布差异有统计学意义(P<0.05),病例组b2等位基因频率(36.64%)明显高于对照组(21.99%)(P<0.001);条件logistic回归分析显示,HSP70、高血压和吸烟是缺血性脑卒中发病的独立危险因素,OR值分别为5.41、2.79和8.17,饮茶则为缺血性脑卒中的保护性因素OR值为0.18。结论HSP70-1突变基因型与缺血性脑卒中易感性有关。

Objective To explore the association between gene polymorphism of heat shock protein 70-1 （HSP 70-1 ） and the susceptibility to ischemic stroke. Methods A 1：1 matched case-control study was conducted. 116 patients with ischemic stroke were selected from two general hospitals in Shenzhen. The controls were selected by the same gender and ethnic group, and each pair＇s ages were permitted to differ within 5 years. HSP70-1 genotypes were determined by using the polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique. The cases and controls were interviewed using the same questionnaire. Results There were significant differences in the distribution of genotypes and allele frequencies between the case group and control group （P 〈 0.05）. The allele frequencies of HSP70-1 b2 in case group （36.64 % ） were higher than those in the control （21.99%） （ P 〈 0.005）. Results of conditional logistic regression demonstrated that HSP70, hypertension and smoking were independent risk factors of ischemic stroke, and their OR values were 5.41, 2.79 and 8.17, respectively. Tea drinking history was a protective factor, and the OR value was 0.18. Conclusion It was suggested that polymorphism of HSP70-1 gene should contribute to the higher risk of ischemic stroke.