盐亭食管癌高发区饮食对人食管癌细胞增殖的影响

Effect of the diet from Yanting region on proliferation of human esophageal cancer cell line

目的采用血清生理学方法,观察盐亭食管癌高发区饮食喂饲大鼠血清对人食管癌细胞Eca-109生长增殖的影响。方法将24只雄性SD大鼠分为3组,分别喂饲大鼠常规饲料、健康成人饮食及盐亭饮食7天,每天定时记录采食量及体重。用MTT法探讨用大鼠血清培养人食管癌细胞的适宜条件;分别以人正常肝上皮细胞HL7702及10%灭活小牛血清作为对照,采用细胞生长曲线、细胞群体倍增时间、3H-TDR掺入实验研究盐亭饮食喂饲大鼠血清对人食管癌细胞生长增殖的影响。结果喂饲健康成人饮食与常规饲料的大鼠其采食量及体重无差异;用5%未灭活大鼠血清取代10%灭活小牛血清适合人食管癌细胞培养;盐亭食管癌高发区饮食喂饲大鼠血清可明显促进人食管癌细胞生长,却不利于人正常肝上皮细胞生长,且与其他3个组均有统计学差异(P<0.05)。结论盐亭食管癌高发区饮食具有促进人食管癌细胞Eca-109生长增殖的作用。

Objective Sero-physlology method was used to observe the effects of sera from rats fed the diet of Yanting （high prevalence region of esophageal cancer） on profiferation of human esophageal cancer cell Eca-109.Methods Male SD rats was submitted to three experimental diets. A standard diet, the healthy adults＇ diet and the Yanting＇s diet were fed for seven days, Body weights and diet intakes were measured every day. Methyl thiazolyl tetrazofium （MTr） assay were used to explore the optimal conditions of rats＇ sofa culturing the cell Eca-109. Human normal liver epithelial cell （HL7702） and 10% deactivated bovine serum were respectively set as normal cell control and serum control. The effects of sora from rats fed the Yanting＇ s diet on Eca- 109 were observed by means of cell growth curve, cell doubling time analysis and ^3H- thymidine incorporation. Results No significant differences of diet intakes and body weights between the rats fed the standard diet and the healthy adult diet were found. It was demonstrated that 5% non-deactivated rats＇ sera could replace 10% deactivated bovine serum in medium for culturing human esophageal cancer cell Eca-109. Contrast to the other three controls, sera from rats fed the Yanting＇s diet significantly promoted Eca-109 cells proliferation, while showed inhibitory effect on human normal liver epithelial cell （HL7702）, Conclusion The results showed that the diet of high prevalence region of Janting esophageal cancer could significantly promote the proliferation of human esophageal cancer cell Eca-109.