江苏地产白首乌C_(21)甾体苷对荷瘤小鼠的免疫保护作用

Immune protecting on tumor-bearing mouse of C_(21) sterols in Baishouwu

目的:探讨白首乌C21甾体苷对肝癌实体型(Heps)小鼠的抗肿瘤作用和免疫保护机制。方法:建立小鼠Heps移植模型,进行抑瘤率计算,对肿瘤病理组织进行观察;计算小鼠的胸腺、脾脏指数;用腹腔巨噬细胞吞噬中性红及分泌NO反映整体免疫功能;MTT法检测T、B淋巴细胞增殖;双抗体夹心ABC-ELISA法检测脾淋巴细胞诱生IL-2及腹腔巨噬细胞诱生TNF-α。结果:C21甾体苷三个剂量组的抑瘤率分别为34.79%、47.08%和50.23%,病理检查可见肿瘤组织中有片状坏死;各剂量组均可降低荷瘤后异常增高的脾指数,升高胸腺指数;增强巨噬细胞吞噬能力;明显增强T、B淋巴细胞增殖反应;使脾细胞分泌IL-2及腹腔巨噬细胞分泌TNF-α量显著提高。结论:白首乌C21甾体苷可提高荷瘤小鼠特异性和非特异性细胞免疫功能,促进抗肿瘤细胞因子的分泌。

AIM： To investigate the anti-tumor effect of the C21 mono-glucosides sterols and its immunity mechanism. METHODS： The models of Heps tumor transplanted in mice were established. The tumor growth inhibition rate （1 R） was calculated, and tumor tissue samples were taken and examined by light microscopy. Spleen and thymus were taken to get spleen index （SI） and thymus index （TI）. The phagocytic ability of peritoneal macrophage to neutral red and the amount of NO were detected to reflect holistic cellular immune level. The T and B lymphocyte transformation capacity were tested by MTI＇ assay. The secretion of interleukin-2 （IL-2） and tumor necrosis factor-alpha （TNF-α） were detected with ABCELISA. RESULTS： After treatment of the C21 steroidal, inhibitory effect on the transplanted Heps was observed in the tuner-bearing mice. The inhibitory rates were 34.79%, 47.08% and 50.23%, respectively. We found the morphology of tumor tissues in C21 groups had typical characters of necrosis under the light microscope. Spleen indices were decreased and thymus indices rose in C21 groups. The C21 steroidal could significantly augment the peritoneal macrophage phagocytic ability. T and B cells proliferation was increased to normal level. IL-2 and TNF-α were elevated when compared with model group. CONCLUSION： The C21 steroidal could improve the specific and non-specific cellular immunity and promote anti-tumor cytokines secretion.