广西五步蛇毒小肽的分离纯化及抗肿瘤作用

Experimental study of a new small peptide purifing from agkitrodon acutus venom on antitumor activities

目的:从广西五步蛇毒分离出小分子活性组分,研究其对肿瘤细胞株的抑制作用。方法:经Sephadex G-75凝胶过滤、超滤和DEAE-Sepharose-CL-6B离子交换层析法从广西五步蛇毒中分离纯化获得一种小分子的肽类,采用MTT法检测其对多种体外培养的癌细胞株的生长抑制情况。结果:从广西五步蛇毒中分离纯化得到的单一的小肽,其对卵巢癌细胞株(A2780)、人胃癌细胞(SGC-7901)以及鼠乳腺癌细胞株(782)有抑制作用并呈明显的剂量依赖关系,作用24h的IC50分别为0.264、0.648、0.173μg/mL。结论:应用凝胶过滤和离子交换层析方法可以从广西五步蛇毒中分离出单一组分的小分子肽,其对多种肿瘤细胞的生长有抑制作用。

AIM： To isolate and purify a new small peptide from Guangxi agkitrodon acutus venom, then investigating its antitumor activity in vitro. METHODS： Small peptide was separated from the snake venom by gel filtration chromatography on superdex 75, ultrafihration and DEAE-Sepharose CL-6B ionexchange. The inhibition on the cultured tumor cells was tested by MTI＇ assay. RESULTS： The peptide purified from Guangxi agkitrodon acutus venom strongly inhibited the proliferation of human ovarian carcimona cell, human gastric cancer cell and mouse breast cancer cell in a dose-dependent manner. The IC5o for 24 h was 0. 264, 0. 648, 0. 173 μg/mL, respectively. CONCLUSION： A peptide can be obtained by gel filtration, ultrafiltration and ionexchange, which exhibits strong effect on inhibiting the proliferation of cultural tumor cells line in vitro.