大鼠急性心梗中血浆瘦素及血流动力学参数变化的探讨

Leptin level change during acute myocardial infarction in rats

目的:研究瘦素在大鼠急性心肌梗死(AMI)过程中的变化及其与心梗发展的关系,从而为AMI的临床诊治提供线索。方法:60只SD大鼠被分为两组,40只用于建立AMI动物模型,为模型组;另20只行假手术,为对照组。在术后1、2、3、4周分别观察这两组大鼠在AMI发展过程中血清瘦素和心肌瘦素的变化,并测定血流动力学参数和心功能的变化,同时测量和计算各阶段心肌梗死面积,以及比较瘦素与各变量间的关系。结果:瘦素在AMI大鼠中呈动态变化,有一个先上升后下降的过程,模型组的瘦素显著高于对照组。在心梗后的四周,瘦素与左室重量指数相关(P<0.05),而与心肌梗死面积的相关性不明显(P>0.05)。模型组大鼠的外周血瘦素与动脉SBP,动脉DBP,左室收缩期峰压(LVESP),左室内压最大上升速率(+dp/dtmax)相关(P<0.05),但与左室舒张末期压力(LVEDP)和左室内压最大下降速率(-dp/dtmax)两参数的相关性不显著(P>0.05)。结论:瘦素参与了大鼠AMI心肌损伤过程,瘦素升高可提示AMI病情恶化。

AIM： Experiments were carried out to explore the variation of leptin level during acute myocardial infarction of SD rats and the inter-relationship between leptin change and AMI progress. METHODS： Sixty Sprague-Dawlaw rats were randomized into 2 groups, 40 rats as the AMI group and 20 rats as the sham-operation group. AMI was experimentally established by ligating left coronary artery anterior descending branch, Leptin level in serum and myocardium was measured at 1st, 2nd, 3rd and 4th week after ligation, respectively. Further, myocardial function and death area, as well as the left ventricle weight index were detected. RESULTS： The leptin level of the AMI group varied during the AMI process, experiencing a period with increasing leptin level at first and then a period with decreasing leptin level. The leptin level of the AMI group is significantly higher than that of the sham-operation group. The myocardial death ＂area for the AMI group did not correlate with serum and myocardial leptin concentrations with a P value 〉 0.05, while the left ventricle weight index correlated with both leptin concentrations in the early phase of AMI with P 〈 0.05. Leptin concentration correlated well with SBP, DBP, LVESP, and ＋ dp/dtmax, with P 〈 0.05, while the correlations of leptin concentration with both LVEDP and - dp/dtmax were not significant, with P 〉 0.05. CON- CLUSION： Leptin is involved in the progress of AMI in the experimental SD rats. An increase in leptin level during AMI may signify myocardial deterioration.