木樨草素抗炎作用机制的初步探讨

Evaluation of anti-inflammatory effects of luteolin and underlying mechanisems

目的:初步评价木樨草素的抗炎作用与机制。方法:采用小鼠足肿胀、肉芽肿和气囊肿模型,考察木樨草素对急、慢性和渗出性炎症的作用;采用脾淋巴细胞增殖实验,观察木樨草素对小鼠的免疫反应的影响;通过人全血分析实验,考察木樨草素对环氧合酶(COX)活性的影响。结果:木樨草素对上述三种炎症反应均有明显抑制作用;能够显著抑制刀豆蛋白A(ConA)刺激引起的小鼠脾细胞增殖,且呈浓度依赖性;对全血COX-1活性无明显影响,但可以显著抑制COX-2的活性。结论:木樨草素可以明显抑制急、慢性炎症反应,其作用机制可能与其对COX-2活性的抑制有关。

AIM： To investigate anti-inflammatory effects of luteolin and underlying mechanisems. METHODS： Three typical inflammatory models, carrageenan-induced paw edema, cotton pellet granuloma and air cyst were used to investigate anti-inflammatory effect of luteolin in vivo. Meanwhile, effects of luteolin on mitogen-induced splenocyte proliferation and cyclooxygenase （COX） activities in vitro were also studied. RESULTS： Luteolin reduced the carrageenan-induced paw edema and effectively inhibited the inflammatory process in the cotton pellet granuloma model. Luteolin significantly inhibited leukocytes influx and reduced elevated levels of PGE2 and 6- keto-PGFla in the exude induced by carrageenan injection. ConA stimulated splenocyte to proliferate, and the response was effectively suppressed by luteolin. In the whole blood assay, luteolin demonstrated selective inhibition of COX-2 activity. CONCLUSION： Luteolin effec tively suppresses inflammatory responses in vivo, and the anti-inflammatory activity is associated with its selective inhibition of COX-2.