FQ-PCR测定HBV DNA与ELISA检测HBV-M相关性分析

A Comparative Study of FQ-PCR and ELISA for the Detection of HBV DNA and HBV-M

目的探讨HBV DNA含量与HBsAg S/CO值之间的相关性。方法分别用荧光定量聚合酶链反应(FQ-PCR)法和酶联免疫吸附试验(ELISA)检测100例乙肝患者的HBV DNA含量和乙肝两对半,并记录HBsAg S/CO值。结果100例乙肝患者HBV DNA含量对数值为(4.28±1.89),HBsAg S/CO值为(15.09±6.64),两指标间作相关性分析,r=-0.0156,P>0.05,无相关性。结论HBV DNA是判断乙肝病毒复制的较准确、较直接的抗乙肝病毒疗效观察指标,而HBsAgS/CO值的高低与疾病的严重程度及疗效无关。

Objective To determine the correlation between the level of HBV DNA and the value of HBsAg S/ CO. Methods 100 serum samples from hepatitis B patients were analyzed for HBV DNA by FQ-PCR and HBsAg by ELISA. The HBsAg S/CO values were also recorded. Results The logarithmic concentration of HBV DNA in these samples was （4.28±1.89） and the HBsAg S/CO value was （15.09±6.64）. There was no correlation between the two values （r=-0.0156, P 〉 0.05 ）. Conclusion HBV DNA is a reliable maker for diagnosis of HBV infection in patients undergoing treatment. There was a poor correlation between the value of HBsAg S/CO and the disease severity.