大鼠正畸移动牙根吸收组织内胶原酶-3的表达与分布

Expression and distribution of MMp-13 in rat root-resorbing tissue induced by orthodontic tooth movement

目的:检测正畸移动牙齿根吸收组织中胶原酶-3(MMP-13)的蛋白表达及其分布,探讨正畸牙根吸收的生物学机制。方法:选用24只SD大鼠建立正畸牙移动根吸收的动物模型,随机分为空白对照组、受力7d组、10d组、14d组。应用免疫组化技术检测并比较各组中MMP-13在第一磨牙根吸收组织中的定位表达,同时比较其在根吸收组织与正常根周组织中表达的差异。结果:MMP-13主要表达于成纤维细胞、成骨细胞、骨细胞及成牙骨质细胞内。但在破骨细胞和破牙细胞内并不显示MMP-13免疫活性。MMP-13在牙根吸收组织中的表达明显高于正常根周组织。结论:MMP-13作为一种重要的蛋白质分解酶可能参与了正畸移动过程中牙根吸收。

Objective：To investigate the expression and the localization of collagenase-3 in root-resorbing tissue and to elucidatethe protein changes and mechanism of root resorption induced by orthodontic tooth movement. Method：24 SD male rats were randomly grouped according to tooth movement days of 0,7, 10 and 14 d. The localization of MMP-13 in rat root-resorbing tissue of the first molar was investigated by immunohistochemistry.Result： MMP-13 was detected in fibroblastic cells, cementoblasts and osteoblasts, but not in odontoclasts or osteoclasts. Moreover, MMP-13 expression was stronglier positive in the root-resorbing tissue relative to the control group.Conclusion： These results suggested that MMP-13 may play an important role in degradation of extracellular matrix in root resorption during orthodontic tooth movement.