摘要
目的:检测正畸移动牙齿根吸收组织中胶原酶-3(MMP-13)的蛋白表达及其分布,探讨正畸牙根吸收的生物学机制。方法:选用24只SD大鼠建立正畸牙移动根吸收的动物模型,随机分为空白对照组、受力7d组、10d组、14d组。应用免疫组化技术检测并比较各组中MMP-13在第一磨牙根吸收组织中的定位表达,同时比较其在根吸收组织与正常根周组织中表达的差异。结果:MMP-13主要表达于成纤维细胞、成骨细胞、骨细胞及成牙骨质细胞内。但在破骨细胞和破牙细胞内并不显示MMP-13免疫活性。MMP-13在牙根吸收组织中的表达明显高于正常根周组织。结论:MMP-13作为一种重要的蛋白质分解酶可能参与了正畸移动过程中牙根吸收。
Objective:To investigate the expression and the localization of collagenase-3 in root-resorbing tissue and to elucidatethe protein changes and mechanism of root resorption induced by orthodontic tooth movement. Method:24 SD male rats were randomly grouped according to tooth movement days of 0,7, 10 and 14 d. The localization of MMP-13 in rat root-resorbing tissue of the first molar was investigated by immunohistochemistry.Result: MMP-13 was detected in fibroblastic cells, cementoblasts and osteoblasts, but not in odontoclasts or osteoclasts. Moreover, MMP-13 expression was stronglier positive in the root-resorbing tissue relative to the control group.Conclusion: These results suggested that MMP-13 may play an important role in degradation of extracellular matrix in root resorption during orthodontic tooth movement.
出处
《临床口腔医学杂志》
2007年第3期145-147,共3页
Journal of Clinical Stomatology