摘要
目的:分析和评价体外培养扩增的骨髓基质细胞(BMSCs)/Bio-oss修复大鼠颅骨极量骨缺损的效果。方法:取同基因大鼠的BMSCs,经体外分离培养扩增,与Bio-oss复合后充填颅骨极量骨缺损。在不损伤硬脑膜的情况下,磨除全层颅骨,造成动物标准缺损,用含或不含BMSCs的Bio-oss充填颅骨缺损。不充填物缺损作为空白对照。术后12周处死动物,取缺损区及周围骨质进行肉眼、X线片及组织学观察。结果:所有植入物无排除或感染。无论X线摄片及组织切片均表明,BMSCs/Bio-oss骨修复能力明显优于的空白组及平片用Bio-oss组。结论:BMSCs-Bio-oss复合物充填相对于单纯材料充填具有明显的骨缺损修复能力,是治疗各种骨缺损较有效的方法。
Objective：The aim of this investigation was to determine whether expanded BMSCs in vitro mixed with Bio-oss could repair critical defects in rat,Method： Bone marrow stem cells from syngeneic rats were cultured in vitro and mixed with Bio-oss to plant the cranial critical size defect, A full-thickness cranicl plate defect was created without damage of dura mater. Bio-oss with or without BMSCs was painted over the cranial defects. Animals being made cranial defect but received no implant served as sham-operated control. A total of 18 impant experiments were carried out, with naked eye examination, X-ray and histologial analysis completed at 12 week.Result：None of the implants exhibited extrusion or infection. Both X-ray pics and histologic slices showed that the bone repair of BMSCS/Bio-oss group 3 was apparently better than that of control group and Bio-oss group.Conclusion. Bio--oss mixed with BMSCs may improve calvarial defects reconstruction more effectively than Bio-Oss alone.
出处
《临床口腔医学杂志》
2007年第3期148-150,共3页
Journal of Clinical Stomatology
基金
国家自然科学基金资助项目(30572065)
