5,4'-二-正辛烷氧基-7-二氟亚甲基异黄酮对人乳腺癌细胞增殖和凋亡的影响

The inhibiting effect of 5,4-Di-n-octoxyl-7-gem-difluoromethylene-genistein on the growth of human breast cancer cells in vitro

目的:探讨金雀异黄素(gen)衍生物5,4'-二-正辛烷氧基-7-二氟亚甲基异黄酮(5,4'-Di-n-octoxyl-7-gem-difluoromethylenegenisteinDOdFMG)体外选择性抑制人乳腺癌细胞增殖和诱导凋亡作用。方法:MTT法检测DOdFMG对MCF-7和HBL-100细胞生长的影响;流式细胞术和AO/EB荧光双染法测定DOdFMG诱导MCF-7细胞凋亡作用及对细胞周期的影响。结果:DOdFMG抑制MCF-7细胞生长,呈时间剂量依赖,比gen具有更强选择性和抗肿瘤活性;DOdFMG能诱导MCF-7细胞凋亡,DOdFMG40.0μM组比gen100μM组诱导凋亡的活性更强,DOdFMGMCF-7将MCF-7细胞阻滞于S期和G2期,呈浓度依赖。结论:DOdFMG能抑制人乳腺癌细胞系(MCF-7)细胞生长和诱导凋亡,是一种高效、毒副作用小的新型治疗乳腺癌候选药物。

Objective： To investigate the effect of the Genistein derivative 5,4＇- Di - n - octoxyl - 7 - gem - difluoromethylene- genistein（DOdFMG） on the growth and apoptosis of human breast cancer（ MCF- 7 ） cell line in vitro. Methods： MTT assay the influence of growth in MCF-7 cells and HBL- 100 cells was examinated; by MTY assay. The apoptosis of MCF - 7 cells was determined by AO/EB double fluorescence stain and PI staining flow cytometry and cell cycle was analyzed using flow cytometry. Results：DOdFMG significantly suppressed proliferation of MCF -7 cells in dose -dependant and time -dependant manner, having the obviously stronger antitumor activity and selectivity than GEN. The apoptosis of MCF -7cells induced and cell cycle was arrested at S stage and G2 stage by DOdFMG in a concentration dependent manner. Compared with GEN 1001μM set, the activity of inducing the apoptosis of DOddFMG 401μM set was stronger. Conclusion： DOddFMG can significantly inhibit MCF - 7 cells growth and induce their apoptosis. It is an efficiently new medicine to treat human breast cancer.