摘要
目的:观察转录因子E2F陷阱DNA对stathmin的表达影响及抑制肿瘤细胞增生情况。方法:采用脂质体LipofectamineTM2000将E2F Decoy ODNs转染入人肺癌A549细胞中,用RT-PCR检测转染细胞中stathmin基因mRNA表达水平变化,通过细胞生长曲线观察转染细胞的增殖能力,Tunel法观察转染细胞凋亡情况。结果:E2F Decoy ODNs被成功转染入肿瘤细胞中,RT-PCR检测结果显示实验组stathmin mRNA表达水平明显低于对照组,而且显著抑制了A549细胞的增殖,空白对照组无明显变化,Tunel凋亡染色可见凋亡细胞。结论:E2F Decoy ODNs能特异性下调stathmin mRNA表达,明显抑制A549细胞的增殖,为肿瘤的基因治疗提供了新的手段。
Objective: To investigate the inhibition effect of E2F decoy Oligodeoxynucleotides (E2F decoy ODNs) in dumbbell shape on mRNA expression of op18 gene and the proliferation of the tumor cells. Methods: E2F decoy ODNs were transfected into A549 cells mediated by lipofectamineTM 2000. The proliferative activity of tumor cells was analyzed by cell grow curve assay, and mRNA expression of op18 gene in tumor cells was detected by RT - PCR. Results: E2F decoy ODNs could be successfully tranfected into tumor cells. The proliferation of tumor cells was inhibited markedly by E2F decoy ODNs. The results of RT - PCR indicated that E2F decoy ODNs decreased expression of op18 gene in tumor cells. Morphologic analysis showed marked increase in apoptosis identified by tunel staining. Conclusion: E2F decoy ODNs may sequence specifically inhibit the activity of transcription factor E2F, inhibit the proliferation of A549 ceils, decrease expression of opl 8 gene, and might be a potential new strategy for tumor gene therapy.
出处
《现代肿瘤医学》
CAS
2007年第4期485-487,共3页
Journal of Modern Oncology