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人CREB4转核机制的初步研究

Primary Study of CREB4 Translocation Mechanism from Cytoplasm to Nucleus
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摘要 cAMP反应元件结合蛋白(cAMP response element-binding proteins,CREB)是一个哺乳动物转录因子家族,通过cAMP反应元件(cAMP response element,CRE)调节cAMP和钙离子依赖性基因的表达.CREB4是CREB转录因子家族的一员.经酵母双杂交筛选人胎脑文库发现CREB4215-279aa可能与核转运因子kayopherinα2相互作用,提示karyopherinα2可能参与CREB4的跨膜转运过程.亚细胞定位结果显示,CREB4全长定位于细胞质,而缺失C端假定转膜结构域的CREB41-279aa蛋白则转移至细胞核内.荧光共定位进一步显示,CREB4和karyopherinα2共定位于细胞质中,CREB41-279aa和karyopherinα2共定位于细胞核中.结果提示C端被切除之后,CREB4被karyopherinα2转运到核内发挥转录作用. cAMP response element-binding（CREB） proteins are a family of mammalian transcription activators that mediate cAMP and calcium-dependent gene expression through the cAMP response element （CRE）. CREB4 is a novel member of the human CREB family. Yeast two-hybrid system revealed that karyopherinα2 may associate with CREB4^215-279aa. Subcellular location of CREB4 and CREB4^1-279aa showed that the GFP-CREB4 fusion protein was localized in cytoplasm, whereas the fusion protein of GFP-CREB4^1-279aa was translocated in nucleus. Our experiments further showed that CREB4 and karyopherinα2 were co-located in cytoplasm, but CREB4^1-279aa and karyopherinα2 were co-located in nucleus. These results suggested that first Cterminal was cleaved and then CREB4was carried into nucleus by karyopherinα2 to activate gene transcription.
作者 刘永强 倪晓华 周丹 王鑫 郭伟伟
机构地区 华东师范大学生命科学学院
出处 《华东师范大学学报（自然科学版）》 CAS CSCD 北大核心 2007年第2期104-110,共7页 Journal of East China Normal University(Natural Science)
基金 上海市自然科学基金(04ZR14040)
关键词 CREB4 酵母双杂交 荧光共定位 kayopherina2 CREB4 yeast two-hybrid fluorescent co-location karyopherinα2
分类号 Q291 [生物学—细胞生物学]
  • 相关文献

参考文献16

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二级参考文献9

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华东师范大学学报（自然科学版）
2007年 第2期

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