6味中药乙醇提取物对B16黑素瘤细胞酪氨酸酶及其相关蛋白-1、-2 mRNA表达的影响

Effects of ethanol extracts from six traditional Chinese medicines on the expression of mRNA for tyrosinase,tyrosinase-related protein-1 and -2 in B16 melanoma cells

目的研究墨旱莲(旱莲草)等6味用于治疗白癜风中药的乙醇提取物对小鼠B16黑素瘤细胞酪氨酸酶及酪氨酸酶相关蛋白-1、-2(TRP-1、-2)mRNA表达的影响。方法半定量逆转录-聚合酶链反应(RT-PCR)测定酪氨酸酶及TRP-1、-2 mRNA的表达,并与阳性对照甲氧沙林(8-MOP)进行比较。结果山慈姑(毛慈姑)、苦参、黑芝麻和墨旱莲的乙醇提取物显著上调细胞酪氨酸酶mRNA的表达(P<0.05);除苦参对TRP-2 mRNA有明显上调作用外(P<0.05),其他中药对TRP-1和TRP-2 mRNA的表达无明显作用(P>0.05)。结论山慈姑、苦参、黑芝麻和墨旱莲有上调酪氨酸酶mRNA的作用,苦参还可上调TRP-2 mRNA的表达。

Objective To study the effects of ethanol extracts from six traditional Chinese medicines for treating vitiligo on the expression of mRNA for tyrosinase （ TYR ）, tyrosinase-related protein （ TRP ） -1, and TRP-2 in B16 melanoma cells. Methods Ethanol extracts were prepared from six traditional Chinese medicines, i.e. Cremastra appendiculata （ D.Don ） Makino, Mentha haplocalyx Briq., Sophora flavescens Air., Prunella vulgaris L., Sesamum indicum L., and Eclipta prostrata L.. B16 melanoma cells were treated respectively with ethanol extracts at a concentration of 10 or 20μg/mL for 72 h, with culture media and 8-methoxypsoralen （ 8-MOP ） served as blank and positive control, respectively. The expression of mRNA for TYR, TRP-1, and TRP-2 was measured by semiquantitative reverse transcription PCR. Results The ethanol extracts from Sophora tlavescens Air. significantly upregulated the expression of mRNA for both TYR and TRP-2 （ both P 〈 0.05 ）. The expression of TYR mRNA was also significantly upregulated by extracts from Cremastra appendiculata （ D.Don ） Makino, Sesamum indicum L., and Eclipta prostrata L. （ all P 〈 0.05 ）, whereas none of the extracts affected TRP- 1 mRNA expression （ P 〉 0.05 ）. Conclusions The results indicate that Cremastra appendiculata （ D.Don ） Makino, Sophora tlavescens Air., Sesamum indicum L. and Eclipta prostrata L. can upregulate the expression of TYR mRNA. Moreover, Sophora flavescens Air can also upregulate TPR-2 mRNA expression. These may partially account for the therapeutic mechanisms of these Chinese herbal drugs in vitiligo.