hTERT蛋白在胃腺癌组织中定量表达的研究

Expression of human telomerase reverse transcriptase in gastric adenocarcinoma

目的探讨hTERT蛋白在胃腺癌组织中表达情况及其与胃癌进展的关系。方法对68例胃腺癌和10例正常组织采用流式细胞术定量检测hTERT蛋白的表达。结果hTERT蛋白流式细胞术测定在进展期胃癌、早期胃癌和正常胃黏膜荧光指数FI值分别为1.92±0.17、1.60±0.18和0.98±0.15,进展期胃癌hTERT含量高于早期胃癌(P<0.01)、早期胃癌高于正常胃黏膜(P<0.01);中、低分化腺癌hTERT蛋白FI值分别为1.89±0.20和1.92±0.19显著高于高分化腺癌1.71±0.19(P<0.01);有淋巴结转移者高于无淋巴结转移者(P<0.05);T1-T2级胃癌TERT蛋白FI值高于T3-T4级胃癌(P<0.05)。结论hTERT高表达与胃腺癌进展相关,可作为胃癌进展的生物学标志之一。

Objectives To discuss the expression level of hTERT protein in human gastric adenocarcinoma tissue and its relationship between hTERT protein and progression of gastric adenocarcinoma. Methods Quantitative expression of hTERTprotein was explored with flow cytometry method in 68 cases of gastric adenocarcinoma and 10 cases of normal gastric mucosa as control. Results FI value of hTERT protein in advanced gastric carcinoma, early gastric carcinoma and normal mucosa were 1.92 ± 0.17,1.60±0.18 and 0.98 ±0.15 respectively with folw cytometry. FI value in advanced gastric carcinoma was markedly higher than that in early gastric carcinoma（ P 〈0. 01） ,and FI value early gastric carcinoma was also increased than that in normal mucosa（ P 〈 0. 01 ）. FI value of hTERT in moderately differentiatied gastric adenocarcinom and poorly differentiated gastric adenocarcinoma were 1.89±0.20 and 1.92 ±0.19,which was distinctly higher than that in normal mucosa 1.71 ± 0.19 （P 〈 0. 01 ）. FI value in lymph node metastasized carcinoma was ascended compared with that of lymph node negative carcinoma. FI value of hTERT of T1 - T1 grade was higher than that of T3 - T4 grade in gastric carcinoma （ P 〈 0. 05 ）. Conclusion There is a correlation between overpressed hTERT protein and gastric adenocarcinoma,hTERT protein maybe a biomarker for gastric progression.