摘要
通过絮凝实验和高岭土表面zeta电位测定，初步研究了agrobacteriumradiobacter菌产生的絮凝剂的絮凝特性和絮凝机理，结果表明阳离子Ca^2＋、Al^3＋、Fe^3＋通过中和高岭土表面的负电荷和增加微生物絮凝剂在高岭土颗粒表面的吸附而促进微生物絮凝剂的絮凝性能；在pH2．0～10．0范围，微生物絮凝剂均有良好的絮凝活性，并且加入微生物絮凝剂后，高岭土的zeta电位曲线下移，表明它对高岭土的絮凝作用不是以电中和为主的，而是以吸附架桥为基础的；不同温度条件下（40～100℃）加热30min，絮凝活性变化很小，表明微生物絮凝剂是表面带阴离子的具有热稳定性的多聚糖类生物大分子，而不是对热敏感的蛋白质类物质，同时紫外扫描光谱也证明了该絮凝剂中不含蛋白质类成分和核酸。
Flocculating mechanisms of bioflocculant from agrobacteriumradiobacter is dealt with in this paper by flocculation tests and measuring Zeta potential of kaolin particle. It is demonstrated that Ca^2＋ , Al^3＋ , Fe^3＋ can improve flocculating efficiency by neutralizing charge on the surface of kaolin particles, and bettering adsorption of bioflocculant on the particles. Zeta potential curve of kaolin particle shifts down at the range of pH2.0 - 10.0 after addition of bioflocculant ;therefore it is proved bioflocculant is partly composed of anion groups and its flocculating function is based on bridging not on the neutralizing charge. The flocculating activity remains almost unchanged when heated for half hour under different temperatures（40 - 100℃ ）. It is showed that the functional component in the bioflocculant is not sensitive to the heat and the ultra-violet spectra is proved that the it is not comprise proteins and nucleic acid.
出处
《矿产综合利用》
CAS
2007年第2期16-19,共4页
Multipurpose Utilization of Mineral Resources
关键词
微生物
絮凝剂
絮凝机理
ZETA电位
Mircroorganisms
Flocculant
Flocculating mechanism
Zeta potential
