钙拮抗剂对3T6成纤维细胞的影响

THE EFFECTS OF CALCIUM ANTAGONISTS ON 3T6 FIBROBLASTS

采用流式细胞技术（ＦＣＭ）观察不同剂量的四种钙拮抗剂（Ｃａ－Ａ）汉防已甲素（Ｔｅｔ）、硝苯啶（Ｎｉｆ）、维拉帕咪（Ｖｅｒ）及脑益嗪（Ｃｉｎ）对３Ｔ６成纤维细胞生长增殖的影响，结果表明它们不仅可阻断Ｇ１→Ｓ期的进程，而且抑制Ｓ期ＤＮＡ的含量，但却增加Ｇ１和Ｇ２期蛋白质的含量，使细胞呈现不平衡生长，最终死亡。其中Ｔｅｔ的作用最为显著，呈一定的量效关系，此作用与阻断钙离子内流无关。提示钙拮抗剂对３Ｔ６成纤维细胞生长增殖的抑制可能是其抗肝纤维化的机制之一。

The effects of calcium antagonists, tetrendrine(Tet), nifedipine(Nif), verapamil(Ver) and cinnacine(Cin) 3T6 fibroblasts, were observed by FCM. The results showed that they can not only block G1→S phase, but also reduce DNA content of S phase, increase protein content of G1 and G2 phases. 3T6 cells will die as soon as possible. In all of the calcium antagonists, Tet has the most remarkable actions and showes the relationship between doses and effects. We conclude that calcium antagonists can inhibit growth and proliferation of 3T6 fibroblasts, and this may be one of its antiliver fibrosis mechanisms.