六神丸逆转白血病细胞多药耐药作用及相关机制研究

Research of Liu Shen Pill Reversing the Multidrug Resistance Effect and Correlation Mechanism of Leukemia Cell

[目的]探讨六神丸逆转白血病细胞多药耐药作用及相关机制。[方法]对急性白血病患者随机分组后,两组病例均继续常规化疗。治疗组予六神丸,90粒/d,分3次服。观察1个月。治疗前后取患者骨髓,吸取单个核细胞,采用免疫荧光法定量测量P-糖蛋白(P170),计算P170蛋白细胞百分比率;用逆转录-聚合酶链反应(RT-PCR)方法对患者的拓扑酶Ⅱα(TopoⅡα)、拓扑酶Ⅱβ(TopoⅡβ)基因进行mRNA水平的检测。[结果]治疗前后组内比较,治疗组P170蛋白表达有所下降,对照组有所上升,但均无显著差异(P>0.01);但两组治疗后组间比较,P170阳性表达例数、阳性表达率以及表达指数,治疗组均明显低于对照组,两者有显著性差异(P<0.01)。TopoⅡβ在治疗前后进行组内比较,治疗组有所上升,对照组有所下降,但均无显著差异(P>0.01);两组治疗后进行组间比较,治疗组明显高于对照组,两者有显著性差异(P<0.01)。TopoⅡα在组间和组内比较均无明显差异。[结论]六神丸可以通过降低P170的表达、提高TopoⅡβ的表达来逆转白血病细胞多药耐药。

[Objective] To discuss Liu Shen pill reversing the multidrug resistance effect and coorrelation mechanism of leukemia cell. [Methods] The patients with acute leucemia were divided treatment group and control group randomly. Two groups were treated with conventional chemotherapy. The treatment group was treated with Liu Shen pill also. To observe for a month. Taking the patient＇s marrow before and after treatment, imbibing the mononuclearceil, P170 was measured quantitatively using immumofluorescence method, then albumin ceil percentage of P170 was calculated; the genes of Tope Ⅱ α and Tope Ⅱβwere examined from the mRNA level using reverse transcription-polymerase chain method （RT-PCR）. [Results] P170 protein expression was decreased in the treatment group and upgraded in the control group before and after treatment, but no remarkable difference （P〉0.01）; the masculine expression number, the masculine expression rate and expression index of P170 in the treatment group were obviously lower than that of in the control group after treatment, there had significance difference （P〈0.01） between two groups. Topo Ⅱ beta was upgraded in the treatment group and decreased in the control group before and after treatment, but no remarkable difference （P〉0.01）; Tope Ⅱ beta in the treatment group were obviously higher than that of in the control group after treatment, there had significance difference （P〈0.01） between two groups. Tope Ⅱ alpha had no obvious difference in the interior-group and intergroup. [Condusion] Liu Shen pill may reversie the multidrug resistance effect of leukemia cell through reducing the expression of P170 and enhancing the expression of Tope Ⅱ beta.