摘要
目的观察861合剂对体外培养贮脂细胞Ⅳ型胶原mRNA的影响。方法与结向传一代的雄性Wistar大鼠肝脏贮脂细胞培养液中加入终浓度为10mg/ml的中药861合剂(0.45μm过滤)或0.625mmol/L的秋水仙素,另设空白对照组,继续培养48小时,收获贮脂细胞提取总RNA,以地高辛配基标记的小鼠Ⅳ型胶原cDNA探针与之做斑点杂交,以β-actin作内对照,结果显示861合剂组及秋水仙素组贮脂细胞Ⅳ胶原mRNA水平较空白对照组明显降低,而且861组比秋水仙素组降低更明显。结论认为861合剂的抗纤维化机理之一是调控贮脂细胞的Ⅳ型胶原表达。
To explore the in vitro effect of herbal compound 861 on type Ⅳ collagen mRNA levels in rat lipocytes. Methods The second passage of lipocytes was exposed to 861 at a final concentration of 10mg/ml, and another group was exposed to colchicine at a concentration of 0.625mmol/L. After 48 hours, the lipocytes were harnested and total RNA was isolated to detrmine type Ⅳcollagen mRNA by dot blot hybridization with digoxigenin labelled cDNA probes. Results The results displayed that type Ⅳ mRNA collagen level in 861 and colchicine treated lipocytes remarkably decreased than that in blank control. Furthermore, the decrease of type Ⅳ collagen mRNA level in 861 treated lipocytes was even more profound than that in colchicine treated lipocytes. Conclusions The anti-fibrotic effect of herbal compound 861 is probably achieved by modulating type Ⅳ collagen expression in lipocytes.
出处
《中华肝脏病杂志》
CAS
CSCD
1996年第3期142-144,共3页
Chinese Journal of Hepatology
基金
卫生部科研课题基金
北京市自然科学基金
关键词
肝纤维化
中药861合剂
Liver fibrosis Medicinal herbs Type Ⅳ collagen