摘要
本实验采用纯系ＳＤ雌性大鼠为实验动物，在背下部伤口上分别于手术后第１，４天，第４，７天，第７，１０天和第１０，１３天，共４组行Ｘ线照射，每次照射６ＧＹ，两次共１２ＧＹ，并分别于手术后第７，１０，２０，４０天取材，在光镜下观察伤口胶原形态学变化。以ＩＢＡＳ－２０００（Ｏｐｔｏｎ）计算机图像分析系统分析胶原密度变化。光镜下观察：胶原纤维排列紊乱，并较细；照射组的胶原平均光密度明显小于对照组。本实验研究提示：通过连续Ｘ线照射后伤口皮肤胶原纤维合成和代谢的抑制作用以手术后第７天内影响最大，第７～１３天的作用也明显高于对照组。通过本实验研究提出了瘢痕疙瘩、增生性瘢痕切除后，在行放射线照射时应以第４，７天为中心。
WeinvestigatedthehistologicalstudyonX-Rayafectingcolagenousfibersduringwoundhealing.WeusedinbredoinefemaleSDratsaslaboratoryanimals.Theyweredividedintofivegroups:fourgroupsunderwentirradiationonthelowerback(irradiatedgroups)respectivelyon1stand4thday,4thand7thday,7thand10thday,10thand13thdayaftertheoperation,andthefifthgroupbeginthecontrolgroup.Theexposuredosewas6GYeachtime.Tissuesweretakenatthewoundarearespectivelyon7thday,10thday,20thdayand40thdayoftheoperation.Weusedlightmicroscopestostudymorphologicalchangesonthesetissues.IBAS-2000Computerizedimagepatternanalysissystem(OPTON)wasusedtoanalysethechangesonthedensityofcolagenousfibers.Onhtelightmicroscopeexamination,colangenousfiberswereirregularandslendered,tissuesfromtheirradiatedgroupsre-vealedadecreasedofthecolagenousfibersdensity.Studyingthehistologicalchangesofthewoundafterfractionatedirradiation,weconcludedthatin7thdayaftertheoperation,X-Rayhasitsmaximalinhibit-ingefectoncolagenousfiberssynthesisandmetabolism.Theefecton7thand10thday10thand13thdayirradiatedgrougsisalsogreaterthanthatofthecontrolgroup.Wesuggestthatincasesofkeloidorhypertrophicscar,Wheneveriradiationtherapyisconsidered,theprotocolshouldbecenteredon4thand7thdayaftertheresection,Wepointedouttheimportanceofanearlierirradiation.
