摘要
从渤海油船泄漏区域的海底泥中筛选到一株能降解柴油的低温解烃菌 T7-2,初步鉴定为红平红球菌(Rhodococcus erythropolis).该菌在接种到以柴油为碳源的海水中,通过补加少量氮、磷盐及微量酵母粉,15℃振荡培养7d,降解率达到60.14%.采用响应面法(RSM)对补加营养进行了优化,方差分析表明二阶多项式模型是适合的,回归分析得到了相应的参数,T 检验证明(NH_4)_2SO_4、Na_2HPO_4和酵母粉均是影响柴油降解的显著性因素,优化的补加营养条件为(NH_4)_2SO_4为2.53g/L、Na_2HPO_4为2.75g/L和酵母粉为10.19mg/L,验证实验和预期一致,优化后降解率达到75.09%,比优化前提高了25%。
A low-temperature hydrocarbons-degrading strain T7-2 was lsolated from seamud of Bohai polluted area and identified as Rhodococcus erythropolis, which could use diesel oil as sole carbon source. Response surface methodology （RSM） was applied to optimize the nutrient amendment to seawater. A five-level three-factor central cofnposite design was employed to determine the maximum degradation at optimum levels for （NH4）2SO4.Na2HPO4 and yeast powder. The respective optimum parameters were 2.53 g/L,2. 75 g/L,and 10. 19 mg/L. The actual experimental results were in agreement with the prediction.
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第1期99-104,共6页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
国家自然科学基金(50674058)
关键词
低温
红球菌
条件优化
响应面法
low-temperature
Rhodococcus sp.
conditions optimization
response surface method
