产苯乳酸的乳酸菌分离筛选及菌种鉴定

Screening and Identification of Phenyllactic Acid-producing Lactic Acid Bacteria

通过对自然发酵泡菜中乳酸菌的分离,建立了一种准确、快速的苯乳酸产生菌的筛选方法。在112株筛选菌株中,获得1株苯乳酸高产菌株SK007。研究了苯丙氨酸对苯乳酸合成的影响,即增加苯丙氨酸的浓度可以提高苯乳酸产量,苯丙氨酸是苯乳酸合成的前体。高产菌株经16S rDNA序列初步鉴定为Lactobacillus sp.,Gen-Bank接受号为DQ534529。系统发育分析表明它与植物乳杆菌(Lactobacillus plantarum)亲缘关系最近。

Screening methods for phenyllactic acid （PLA）--producing lactic acid bacteria was developed, One strain, SK007, which was isolated from Chinese traditional pickles, showed the highest PLA--produ- cing ability among 112 strains. Furthermore, information gathered by supplying the strain SK007 with phenylalanine （Phe） indicated that PLA yield is improved using increased concentrations of Phe and Phe is the precursors of PLA. The screened strain was identified as Lactobacillus sp. by 16S rDNA sequence. The 16S rDNA sequence of Lactobacillus sp. SK007 has been deposited in GenBank under accession number DQ534529. Phylogenetic analysis showed that it closely related with Lactobacillus plantarum .