灰绿曲霉突变株降解稻草秸秆及联合产氢的研究

Degradation of Rice Straw by Aspergillus glaucus Mutant and integration with Biohydrogen Production

对灰绿曲霉（Aspergillusglaucus）突变株EU7—22进行单因子和正交实验，确定菌株利用稻草产纤维素酶的液态发酵优化组合条件为：稻草粉3％、蛋白胨0．5％、温度35℃、起始pH5。在优化条件下培养4～5d可达到产酶高峰。利用优化培养后的酶液水解稻草粉产糖，在最适条件下，稻草粉酶解反应24h后，糖化率可达4．9％。利用稻草粉做底物，EU7—22和产氢克雷伯氏菌HP1联合发酵，1g稻草粉可产氢23．7mL。

The optimal conditions for cellulase production of Aspergillus glaucus mutant strain EU7-22 was achieved as follows, rice straw powder quantity 3%, peptone quantity 0.5%, original pH 5, and temperature 35℃, by orthogonal experiments. The strain reached its maximum cellulose activity after 4 ~ 5 days fermentation. When the cellulase was applied for saccharification of rice straw, a saccharification rate of 49 % was obtained after 24h hydrolysis. The saccharification liquid was subsequently used for hydrogen production. A yield of 23.7 mL H2/g rice powder was acquired when using Klebsiella oxytoca HP1 as the hydrogen producer.