羊心脏束细胞-心肌细胞演化系观察

Observation of bundle cell-cardiomyocytic developing strain in goat ventricle

目的:观察羊心室内膜下层束细胞演化形成心肌细胞的动态过程。方法:12只山羊取心脏后,常规石蜡切片,HE染色,光学显微镜观察。结果:正常成体羊心室内膜下Ⅰ型束细胞分裂增生为Ⅱ型束细胞团或心肌生成单位。生成单位经横向与纵向2种方式,演化形成蒲肯野纤维和工作心肌细胞;部分Ⅰ型束细胞经Ⅱ型束细胞直接演化为工作心肌。结论:正常成体羊心室传导心肌和工作心肌属同一细胞演化系,羊心室心肌主要来源于束细胞-心肌细胞演化系。

Aim： To study the dynamic process of bundle cells evolving into cardiomyocytes in subendocardial layer of gaot ventricle. Methods：The paraffin sections of hearts from 12 goats, stained with HE, were observed under light microscope. Rosults： In normal adult goat ventricle, subendocardial type Ⅰ bundle cells proliferated and evolved into Type Ⅱ bundle cells or cardiomyocyte forming unit （CMFU）. The CMFU evolved into Purkinje fiber and working cardiac muscle by transverse and longitudinal pattern. Some of Type I bundle cells evolved into working cardiac muscles directly via Type Ⅱ bundle cells. Conclusion： In normal adult goat ventricle, conduction and working cardiac muscles were in same evolving system, and the ventricular muscles were mainly originated from the bundle cells-cardiomyocytic developing strain.