喉鳞状细胞癌组织中血管内皮生长因子和环氧化酶-2 mRNA检测

Expression of VEGF and COX-2 mRNA in laryngeal squamous cell carcinoma tissue

目的:探讨喉鳞状细胞癌组织中血管内皮生长因子(VEGF)和环氧化酶(COX-2)mRNA的表达情况及与癌组织生长、浸润及转移的关系。方法:应用RT-PCR方法检测62例喉鳞状细胞癌、54例癌旁和9例正常喉黏膜组织中VEGF mRNA及COX-2 mRNA的表达。结果:喉鳞癌组织中VEGF mRNA和COX-2 mRNA的表达量高于癌旁和正常喉黏膜组织(P<0.01),Ⅰ、Ⅱ期喉鳞癌组织2者的表达量低于Ⅲ、Ⅳ期(P<0.01)。喉鳞癌组织中VEGF mRNA和COX-2 mRNA的表达呈正相关(r=0.756,P<0.01)。结论:VEGF和COX-2与喉鳞癌的生长、浸润及转移有密切关系,2者有协同作用。

Aim ： To study the expressions of vascular endothelial growth factor （VEGF） and cyclooxygenase-2 （ COX- 2） mRNA in human laryngeal squamous cell carcinoma （LSCC） tissue and its significance. Methods： The expression of VEGF mRNA and COX-2 mRNA in 62 cases of LSCC, 54 cases of adjacent to tumor tissue, and 9 normal human laryngeal mucous membrane were detected using techniques of semi-quantify RT-PCR. Results： The expression level of VEGF mRNA and COX-2 mRNA in LSCC tissue were significantly higher than those in the normal human laryngeal mucous membrane and adjacent to tumor tissue （P 〈 0.01 ）. The expression level of VEGF mRNA and COX-2 mRNA in Ⅲ and Ⅳ stage tissues were significantly higher than those of Ⅰ and Ⅱ stage tissues of LSCC （P 〈0.01 ）. There was a high positive correlation between VEGF and COX-2 mRNA expression in LSCC （r = 0. 756,P 〈 0.01 ）. Conclusion： VEGF and COX-2 may play key roles in the growth, invasion and metastasis of LSCC.