酪氨酸激酶抑制剂STI571对K562细胞生长和凋亡的影响

Effect of tyrosine protein kinase inhibitor STI571 on growth and apoptosis of K562 cells

目的:探讨酪氨酸激酶(TPK)抑制剂STI571对K562细胞生长和凋亡的影响。方法:取对数生长期K562细胞,分4组培养。Ⅰ组不加任何药物,Ⅱ组、Ⅲ组、Ⅳ组分别加0.5μmol/L、1μmol/L和2μmol/L的STI571,继续培养5d。每d行细胞计数,台盼蓝拒染法检测细胞活力,连测5d。培养36h时,取细胞,双缩脲法检测细胞蛋白含量,采取蛋白免疫印迹法进行Procaspase-3及Bcl-xL检测。结果:Ⅱ组、Ⅲ组细胞数低于同一时间段Ⅰ组细胞数(P<0.05),但高于Ⅳ组细胞数(P<0.05)。与Ⅰ组比较,Ⅱ组、Ⅲ组和Ⅳ组Procaspase-3和Bcl-xL蛋白表达降低(P<0.01);与Ⅱ组和Ⅲ组比,Ⅳ组2者表达量更低(P<0.01)。结论:STI571通过下调Bcl-xL,激活Procaspase-3,诱导K562细胞凋亡,从而抑制K562细胞的生长。

Aim. To explore the effect of tyrosine protein kinase inhibitor STI571 on growth and apoptosis of K562 cells. Methods：K562 cell line were divided into 4 groups, Group Ⅰ was the control, Group Ⅱ ,Group Ⅲ ,Groupiv were cultured with 0.5 μmol/L, 1 μmol/L, and 2 μmol/L STI571, respectively, for 5 days. The numbers of cells were recorded every day, and the expression of Bcl-xL and Procaspase-3 of cells cultured for 36 h were studied by Western blot. Results： The numbers of Group Ⅱ , Group Ⅲ were higher than that of Group I , but lower than that of group IV （ P 〈 0.05 ）. Compared with group Ⅰ , the expression of Procaspase-3 and Bcl-xL of group Ⅱ , group Ⅲ and group IV were lower（P 〈 0.01 ） , and the expression of group IV was lower than those of Group Ⅱ and Group Ⅲ （ P 〈 0.01 ）. Conclusion ： STI571 can suppress the expression of Bcl-xL and Procaspase-3, and inhibit the growth of K562 cell.