河南汉族胃癌患者IL-1B和IL-1RN基因多态性分布特征

Relationship between genetic polymorphisms of IL-1B,IL-1RN and gastric cancer in Henan Han population

目的：研究河南汉族胃癌患者白细胞介素（IL）-1B和IL-1RN基因多态性的分布特点。方法：以PCR-限制性片段长度多态性（PCR．RFLP）分析法对河南汉族65例胃癌患者和130例非胃癌患者的IL-1B-31、IL-1B-511及IL-1RN基因位点的多态性进行分析。结果：胃癌患者IL-1B-31C／C和IL-1B-511T／T位点基因型频率高于对照人群（OR分别为6．6（95％CI=1．2～3．5）和3．3（95％CI=1．1～10．1））；但IL-1 RN各基因型频率2组间差异无统计学意义（P〉0．05）。结论：IL．1B-31C／C和IL-1B-511位点T／T基因多态性可能与河南地区胃癌的发生有关。

Aim ： To study the distributions of the frequencies of interleukin-1 B（ IL-1 ） and IL-1RN gene polymorphism in Han population in Henan Province. Methods：The genetic DNA was extracted from the peripheral blood of 130 individuals and 65 gastric cancer patients in Henan Province. IL-IB and IL-1RN gene polymorphism were analyzed using PCR-restriction fragment length polymorphism. Results： Compared with those of the control, the frequencies of IL-1B-511 T/T genotype and IL-1 B-31 C/C genotype in patients with gastric cancer were significantly higher（ OR was 3.3 （95% CI = 1.2 3.5 ） and 6.6 （95% CI = 1.2 -3.5 ） , respectively） , While all kinds of IL-1 RN genotype frequencies of patients with gastric cancer didnt differ from those of the control （P 〉0.05）. Conclusion： IL-1B-31 C/C and IL-1B-511 T/T gene polymorphism may be risk factors of gastric cancer in this area.