间接白蛋白钴结合试验测定血清缺血修饰白蛋白

Detection of ischemia modified albumin by albumin cobalt binding test

目的：采用国产试剂盒，利用间接白蛋白钴结合试验对血清缺血修饰白蛋白（IMA）进行自动分析测定。方法：用HITACHI公司7600-020全自动生化分析仪和国产试剂盒，用间接白蛋白钻结合试验测定100名正常人血清IMA含量。结果与结论：本法批内变异1．31％，批间变异3．30％，最低检出限10～18ku／L，线性范围14～200ku／L，白蛋白质量浓度在20～50g／L范围内时与IMA浓度呈线性关系，Y=4．58X-30．8（r=0．999，P〈0．01）。本法测定血清IMA正常值范围〈70．0ku／L。

Aim： To measure serum ischemia modified albumin（IMA） level using albumin cobalt binding （ACB） test with domestic reagent. Methods：Serum level of IMA of 100 healthy individiuls were detected by ACB test using HITACHI- 7600 autobiochemical analyzer. Results and Conclusion： Inter-experimental reproducibility for sample were 1.31% and 3.30% , respectively,the detection limit was 10 -18 ku/L, the linear range was 14 -200 ku/L. The contents of albumin and IMA had linear relation within 20.0 g/L to 50.0 g/L, Y = 4.58X - 30.8 （ r = 0. 999 ,P 〈 0.01 ） , and the reference value of normal people was less than 70.0 ku/L.