摘要
目的观察氯化汞(HgCl2)对胫神经的影响。方法SD大鼠30只,雌雄各半,体重160～200g。随机分为3组,每组10只。大剂量染汞组按17mg/kg(1/4 LD50);小剂量染汞组按8.5mg/kg(1/8 LD50),经口灌胃HgCl2溶液,每天1次。对照组经口灌胃生理盐水2ml/次,每天1次,三组均连续灌胃(20±2)d。染汞组制成亚急性汞中毒模型后,用二巯丙磺钠(DMPS)驱汞2个疗程(14d)。分别于驱汞前和驱汞后分批处死大鼠,取其胫神经并用透射电镜观察超微结构的变化。结果大剂量染汞组中有7只出现汞中毒,其中1只雄鼠出现疼痛表现。小剂量组中有6只出现汞中毒,均无疼痛表现。电镜放大4000至20000倍见:汞中毒大鼠胫神经出现脱髓鞘和轴索病变,DMPS驱汞2个疗程未能阻止周围神经病变。结论亚急性HgCl2中毒可导致SD大鼠胫神经损伤。
Objective To observe the effect of mercury on the peripheral nervous system. Methods Thirty SD rats （15 ♀,15 ♂, weighting 160 - 200 g） were randomized into 3 groups. The rats in three groups were given HgCl2 juice at a dose of 0 （negative control, 0.9% saline 2 ml ）, 17mg/kg （1/4 LD50） and 8.5mg/kg （1/8 LD50） by gavage daily, respectively. After rats received HgCl2 juice for 20±2 days, the dosed rats was given the chelator （DMPS） 28mg/kg by intraperitoneal injection for 14 days. Then the rats were dissected and the nerve of tibialis was observed by electric microscope （magnification 4000 - 20 000 times）. Results Seven rats in high dose group were poisoned and one of them had pain manifestation. Six rats in the low dose group were poisoned. The tibialis nerve of poisoned rat was myelioclasis. DMPS did not stop the illness casued by mercury poisoning. Conclusion Sub-acute poisoning of HgCl2 result in injure of tibialis nerve of SD rat .
出处
《职业卫生与应急救援》
2007年第1期10-13,共4页
Occupational Health and Emergency Rescue
关键词
汞中毒
疼痛
周围神经
脱髓鞘
轴突
Mercury poisoning
Pain
Peripheral nervous system
Myelinoclasis
Axon.
