偏头痛肝阳上亢证大鼠肾上腺蛋白质双向电泳图谱的分析

Analysis of two-dimensional gel electrophoresis profiles of the rat Adrenal glands of migraine with excessive rising of liver-YANG

目的探寻偏头痛肝阳上亢证大鼠相关蛋白。方法将SD大鼠随机分成正常组及偏头痛肝阳上亢证组（简称模型组）。通过对肾上腺组织蛋白质的提取，经双向电泳，Pdquest软件分析，蛋白质点原位酶解及质谱分析。结果发现模型组与正常组大鼠肾上腺总蛋白质的分布模式非常相似，以PI3—8，分子量Mr14．4-75kD范围的蛋白质斑点分布最多。经PDQuest7．0软件分析后得出，在相同条件下识别的蛋白质斑点数分别为正常组（584±25）个，模型组（686±27）个。选取表达有显著差别的12个蛋白质斑点进行酶解和质谱分析，所得结果在网上搜寻，其中有9个点的蛋白质与之匹配。结论表明模型组与正常大鼠肾上腺蛋白质的表达具有差异，经质谱鉴定的9个蛋白质可能与偏头痛肝阳上亢证的形成有关．这为进一步研究偏头痛发病机制打下了基础。

[Objective] To explore protein associated with migraine with excessive rising of liver-YANG. [Methods] SD rats were derided into the normal group and the migraine with excessive rising of liver-YANG group （the mode group） randomly. Adrenal glands proteins were extracted and then further analysed by two-dimensional gel eleetrophoresis, PDQuest 7.0 software analysis, In2gel protein digestion and mass spectrogram analysis. [Result] Total protein distribution patterns of the model group and the normal group were very similar. Protein spots were mainly displayed at pI3-8, Mr14.4-75 kD range, through PDQuest 7.0 software analyzing, and there were （686±27） protein spots in the mode group while （584±25） spots in the normal group. Take 11 protein spots that were notable expressed to digest and MALDI-TOF-MS analyze.Compared with the results found on the intemet, 8 protein spots were matched. [Conclusion] There are differences in the model group and the normal Adrenal glands. These 8 protein spots may be concerned with the formation of migraine with excessive rising of liver-yang. This result will pro- vide foundation for identifying the pathogenesy of migraine.