血清髓过氧化物酶和高敏C反应蛋白与冠状动脉粥样硬化的相关性研究

The correlation of serum myeloperoxidase and high sensitivity Creactive protein with coronary artery atherosclerosis

目的探讨血清髓过氧化物酶(MPO)和高敏C反应蛋白(hs-CRP)在冠状动脉粥样硬化不同程度中的变化。方法应用酶联免疫吸附试验(ELISA)测定67例经冠状动脉造影证实的冠心病患者和20例造影阴性对照者的MPO水平,冠心病患者按造影结果分为3组。用免疫透射比浊测定hs-CRP,同时检测血清中的总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)的水平以及白细胞(WBC)计数等指标。结果造影阴性组MPO水平为(4.53±1.26)U/mL,病例组中狭窄程度≤50%为(4.35±1.12)U/mL,50%<狭窄程度≤75%为(3.57±0.77)U/mL,狭窄程度>75%为(2.92±0.47)U/mL。除狭窄程度≤50%组外,其他病例组血清MPO水平均显著低于造影阴性组。hs-CRP则与之相反。MPO水平与WBC计数呈正相关(r=0.389,P=0.003)。结论MPO在血清中的低水平表达可作为冠状动脉粥样硬化的危险因子,是独立于hs-CRP的炎症标志物。

Objective To investigate the changes of serum myeloperoxidase （MPO） and high sensitivity C-reactive protein （hs-CRP） in coronary artery atherosclerosis. Methods The serum levels of MPO were determined by enzymelinked immunosorbent assay （ELISA） in 67 patients diagnosed as coronary artery atherosclerosis by angiography and 20 cases which angiography was normal as control group. The patients were divided into three groups according to angiography result. Immuno-turbidimetry was applied to measure the level of serum hs-CRP. Total cholesterol, triglyceride,low density lipoprotein cholesterol,high density lipoprotein cholesterol and white blood cell （WBC） count were measured. Results The serum level of MPO in stenosis group were significantly lower than in control group,except patients which degree of vascular stenosis ≤〈50%. The level of MPO in patient with angiography negative was （4.53 ± 1.26）U/mL; the patient with the degree of vascular stenosis ≤50% was （4.35 ± 1.12） U/mL;the patient with the degree of vascular stenosis 50% - 75% was （3.57 ± 0.77） U/mL; the patient with the degree of vascular stenosis 〉 75% was （2.92 ± 0. 47） U/mL. The serum levels of MPO were not correlated with hs-CRP but positively correlated with WBC count （r =0. 389,P = 0. 003 ）. Conclusions The low expression of MPO in serum may be a risk factor of coronary artery atherosclerosis and it is an inflammation marker independent of hs-CRP.