乌头类中药对CHL细胞的DNA损伤作用

STUDY ON DNA DAMAGE IN CHL CELLS CAUSED BY ACONITE

[目的]观察乌头类中药盐附子和乌头碱对CHL细胞(中国仓鼠肺成纤维细胞)的DNA损伤作用。[方法]采用单细胞凝胶电泳法检测盐附子、乌头碱对CHL细胞DNA即刻损伤的影响。在加和不加S9时,盐附子均设5个剂量组(终浓度分别为5、2.5、1.25、0.625、0.313 mg生药∕ml)、乌头碱设4个剂量组(终浓度分别为100、50、25、5μg/ml),试验同时设PBS阴性对照组和阳性对照组,阳性对照在不加S9时为0.1 mmol/ml重铬酸钾,加S9时为80μg/ml环磷酰胺。[结果]在加和不加S9时,盐附子各浓度组拖尾细胞率和拖尾细胞尾长与阴性对照比较差异无统计学意义(P﹥0.05),乌头碱100μg/ml和50μg/ml组拖尾细胞率和拖尾细胞尾长与阴性对照之间差异有统计学意义(P﹤0.05),乌头碱各浓度组拖尾细胞率和拖尾细胞尾长存在剂量反应关系。[结论]在本试验条件下,加与不加S9时,乌头碱浓度为100μg/ml和50μg/ml时对CHL细胞有DNA损伤作用;盐附子5、2.5、1.25、0.625、0.313 mg生药/ml时未观察到对CHL细胞有DNA损伤作用。

[Objective] To observe DNA damage in CHL cells caused by aconite. [Methods] The DNA damage in CHL cells processed by salted aconite root extractive and aconitine by SCGE was detected. There were five groups of salted aconite mot extractive （the final concentrations were 5, 2.5, 1.25, 0.625 and 0.313 mg raw drug/ml respectively）, and four groups of aconitine （the final concentrations were 100, 50, 25 and 5 μg/ml, respectively） . The PBS negative and positive control group were set up, while in the positive Control group there were 0.1 mmol/ml bichromicum kalium with S9 and 80 μg/ ml cyclophosphamide without S9. [ Results] The rate of tailing cell and the length of tailing cell in each concentration of salted aconite root extractive group had no significant difference compared with the negative control group with S9 or without S9 （P 〉 0.05） . The rate of tailing cell and the length of tailing cell in aconitine group had significant difference in 100 μg/ml and 50 μg/ml group compared with the negative control group, The dose-respose relationship was observed in aconitine group with or without S9. [Conclusion] Aconitine can damage DNA of CHL cell at the level of 100 μg/ml and 50 μg/ml, while salted aconite root had no such DNA damage at the level of 5, 2.5, 1.25, 0.625 and 0.313mg raw drug / ml.