ox-LDL诱导血管内皮细胞表达PECAM-1的研究

STUDY ON ox-LDL-INDUCED EXPRESSION OF PECAM-1 IN VASCULAR ENDOTHELIAL CELLS

[目的]控讨ox-LDL致动脉粥样硬化作用的分子机制。[方法]体外培养人脐静脉内皮细胞(HU-VEC),ox-LDL作用于血管内皮细胞后,采用逆转录聚合酶链反应(RT-PCR)技术检测PECAM-1 mRNA表达,采用Western印迹法检测PECAM-1蛋白表达。[结果]在培养的HUVEC,ox-LDL在50 mg/L～200 mg/L浓度范围内呈浓度依赖性上调PECAM-1基因转录和蛋白表达。[结论]ox-LDL可在转录水平上调血管内皮细胞PECAM-1基因表达,从而促进血管内皮细胞高水平表达PECAM-1。

[Objective] To investigate the effect of oxidized low-density lipoprotein （ox-LDL） on the expression of PECAM-1 mRNA and protein in cultured human endothelial ceils. [ Methods] Effects of ox-LDL on expression of platelet endothelial cell adhesion molecule-1 （PECAM-1） protein and mRNA in human umbilical vein endothelial cell （HUVEC） were determined with western blotting and reverse transcription polymerase chain reaction （RT-PCR） . [ Results] ox-LDL up-regulated PECAM-1 mRNA expression and protein synthesis in HUVEC in concentration-dependent manner after incubating with 50 mg/L, 100 mg/L and 200 mg/L LDL [ （0.54 ± 0.08 vs 0.85 ± 0.07, 1.56 ± 0.08, 1.39 ± 0.20; P 〈 0.05）, （880 ± 90 vs 2470 ± 440, 2930 ± 470, 2850 ± 620; P 〈 0.05）] . [Conclusion] ox-LDL can up-regulate the expression of PECAM-1 gene at transcription level and increase the expression of PECAM-1 protein in HUVECs.