摘要
[目的]从自发活动、肌肉耐力和学习记忆3方面了解生草乌对小鼠神经系统的毒性作用。[方法]试验设立阴性对照组、阳性对照组和生草乌27.48 g生药/kg、13.74 g生药/kg、6.87生药g/kg 3个试验组。测试指标:①小鼠自发活动数:单次给药15 min、30 min和3 h后用自发活动仪测定小鼠在10 min内自发活动次数。②爬杆时间:单次给药30 min后将小鼠置于爬杆架的玻璃棒上,记录小鼠由于肌肉疲劳从棒上跌落的时间,累计3次作为爬杆时间。③平均逃避潜伏期:连续给药4 d,末次给药后30 min进行水迷宫实验,以小鼠找到隐藏平台的时间为逃避潜伏期,测试两次取平均值。通过比较实验组和阴性对照组以上3个测试指标,了解生草乌对小鼠神经系统的毒性作用。[结果]采用单因素方差分析和最小有意义差异法(LSD法)比较生草乌低、中、高3个剂量组和阴性对照组之间小鼠的3个测试指标,结果显示:各给药组与阴性对照组之间小鼠自发活动数、爬杆时间和平均逃避潜伏期的差异均无统计学意义(P﹥0.05)。[结论]生草乌低、中、高3个剂量组与阴性对照组相比,在自发活动、肌肉耐力和学习记忆3方面的差异均无统计学意义,故认为在本实验条件下,生草乌剂量为0.229、0.458、0.916 g生药/ml时,对小鼠精神神经系统无毒性作用。
[Objective] To study the neurotoxicity of Radix Aconiti Kusnezoffi in mice. [ Methods] Divide the 50 Kunming mice were divided into five groups, which represent one negative control group, one positive group and three dosing （0.229, 0.458, 0.916graw drug/ml） groups. The capability of acquiring memories, resistance of muscle and spontaneous activities were observed in the three separate tests. [Results] There was no significant defference between the dosing groups and negative control group in the capability of acquiring memories, resistance of muscle and spontaneous activities （P 〉 0.05） . [Conclusion] Radix Aconiti with 0.229, 0.458, 0.916graw drug/ml have no effects on the capability of acquiring memories, resistance of muscle and spontaneous activities.
出处
《现代预防医学》
CAS
北大核心
2007年第7期1218-1220,共3页
Modern Preventive Medicine
基金
国家自然科学基金资助项目(30230410)
