正己烷对实验大鼠肝细胞凋亡的影响

EFFECTS OF N-HEXANE ON HEPATIC CELL APOPTOSIS IN EXPERIMENTAL RATS

[目的]研究正己烷(n-Hexane)对染毒大鼠肝细胞凋亡的影响。[方法]将40只雄性SD大鼠随机分成5组,即阴性对照组、75、150、300 mg/kg染毒组和阳性对照组(环磷酰胺),每组8只。经腹腔注射染毒4周后,观察大鼠的一般状况和体重变化,用流式细胞仪检测肝细胞凋亡情况。[结果]大鼠体重随染毒时间而增加,阴性对照组增加最快;流式细胞仪检测结果显示,肝细胞凋亡率(AV+/PI-)组间比较差异有统计学意义(P﹤0.01),与染毒剂量作相关分析,相关系数为0.913,无明显的相关性(P﹥0.05)。[结论]正己烷可引起肝细胞凋亡和坏死比例的增加。

[Objective] To study the effect of n-Hexane on hepatic cell apoptosis in rats. [Methods] 40 SD male rats were randomly divided into 5 groups, namely groups of negative control, 75, 150, 300 mg/kg and positive control （cyclophosphamide ） . Rats were administrated with n-Hexane through intraperitoneal injection. After the dosages were given according to the weight of rats for four weeks, the rats＇ general status and the changes of its weight were recorded to detect apoptosis rate of the hepatic cell by FCM. [Rosults] The body weight of the rats increased in each group with time and it increased more quickly in the negative control group than others. The detected apoptosis rate of the hepatic cell by FCM anlong the groups were statistically significant, no correlation between dosage and apoptosis rate was found （r = 0.913, P 〉 0.05） . [Conclusion] n-Hexane can increase the proportion of apoptosis and cellular necrosis of the rats＇ hepatic cell.