多层膜干片法测定血清酒精浓度的方法学探讨

MULTI-LAYER DRY SLIDES METHOD IN THE DETERMINATION OF SERUM ALCOHOL

[目的]了解多层膜干片法测定血清酒精浓度的试验特点。[方法]采用多层膜干片法测定血清酒精浓度,并对其方法学进行初步研究。[结果]批内重复性实验的平均变异系数为1.91%;批间重复性实验的平均变异系数为2.12%;平均回收率为102.00%;线性达290 mg/dl(Y=0.97X-2.45,r=0.997);加入胆红素﹤342.08μmol/L,血红蛋白﹤5 g/L,甘油三酯﹤18.08 mmol/L无显著干扰。该法与免疫荧光偏振法测定结果呈高度相关,r为0.998。[结论]本方法结果重复性好,线性范围宽,准确,快速,适合临床应用。

[ Objective] To understand the characteristics of multi-layer dry slides method in the determination of serum alcohol. [Methods] Serum alcohol was determined by multi-lay dry slides method. [Results] In repetitive experiment, the average coefficient of variation within-run, between-run and the average recovery ratio was 1.91%, 2.12% and 102.00% respectively. The linearity reached 290 mg/dl （Y= 0.97X-2.45, r = 0.997） . After adding Bilirubin 〈 342.08 μmol/L, Hemoglobin 〈 5 g/L, Triglyceride 〈 18.08 mmol/L, there was no significant difference. The detected result by this method was highly corrlated with that by Fluorescence Polarization lmmunoassay method, the correlative coeffieient was 0.998. [ Conclusion ] This method has the advantages of accurate results, broad linearity, good repetition, and rapid report for clinical application, which can be used in clinical practices.