GDX-502树脂富集高效液相色谱法测定地表水中酚类化合物

High Performance Liquid Chromatographic Determination of Phenol Compounds in Ground Surface Water Samples with GDX-502 Resin Concentration

采用自制GDX-502固相萃取小柱萃取水中苯酚、对硝基酚、间甲酚、2,4-二氯酚、2,4,6-三氯酚、五氯酚等6种酚类化合物,以乙腈作为洗脱剂,选择280 nm和290 nm双波长紫外高效液相色谱进行检测。结果表明,方法检出限为0．01～0．05 mg/L,1L水的平均加标回收率为82．5%～95．0%,精密度(RSD,n=7)为3．6%～9．2%。方法用于实际地表水中痕量酚类化合物的检测,操作简单,有机溶剂用量少,分析效率高;萃取小柱可多次重复使用,分析成本低。

A method for the determination of phenol compounds in ground surface water samples by high performance liquid chromatography （HPLC） was developed. The phenol compounds including phenol, paranitrophenol, metacresol, 2,4-dichlorophenol, 2,4,6-trichlorophenol and pentachlorophenol are extracted with GDX-502 resin as solid phase extraction column and eluted with acetonitrile and then determined by HPLC with double-wavelength （at wavelength of 280 nm and 290 nm） ultraviolet detector. The results show that the detection limit of the method is 0.01 - 0.05 mg/L for these phenol compounds, the average recovery for 1 L water samples is 82.5% -95.0% with precision of 3.6% -9.2% RSD （n =7）. The method has been applied to the determination of trace phenol compounds in practical ground surface water samples with the advantages of simplicity, less organic solvent consumption, high efficiency and low cost.