环维黄杨星-D对大鼠局灶性脑缺血再灌注损伤的影响

Effect of cyclovirobuxinum-D on cerebral ischemia-reperfusion injury in rats

目的:观察环维黄杨星-D(CVB-D)对大鼠局灶性脑缺血再灌注损伤的保护作用,并探讨其作用机制。方法:取大鼠120只,随机分为6组,即CVB-D高、中、低剂量组(2,1,0.5 mg.kg-1),尼莫地平组(2 mg.kg-1),模型对照组和假手术组,每组20只。采用线栓法建立大鼠大脑中动脉阻断再灌注模型,观察CVB-D对模型大鼠神经损伤症状、脑梗死范围、脑组织超氧化物岐化酶(SOD)活性、丙二醛(MDA)含量、脑细胞内钙离子(Ca2+)浓度及脑组织病理变化的影响。结果:CVB-D高、中剂量能显著改善局灶性脑缺血大鼠神经症状,提高脑组织SOD活性;CVB-D高剂量能降低脑组织梗死范围、MDA含量及脑细胞内Ca2+浓度;CVB-D各剂量组均能减轻脑组织病理变化。结论:CVB-D对大鼠局灶性脑缺血再灌注损伤具有保护作用。

Objective： To observe the effect of cyclovirobuxinum-D （CVB-D） on cerebral ischemia-reperfusion injury in rats and explore its mechanisms. Method： One hundred and twenty rats were randomly divided into three CVB-D groups （ 2, 1, 0. 5 mg · kg^-1 ） , Nimodipine group （2 mg · kg^-1 ）, model group and sham operated group, 20 rats each group. Rat cerebral ischemia-reperfusion injury model was induced by middle cerebral artery occlusion, the nerve injury symptoms was evaluated, the level of SOD and MDA in brain tissue were determined, the concentration of intracellar Ca^2 ＋ of brain was measured, and the pathological change of brain was also observed. Result： CVB-D could improve the nerve injury symptoms, reduce the infarction area of brain, the concentration of intracellar Ca^2 ＋ and the level of MDA, increase the activity of SOD, and decrease the pathological change of brain. Conclusion： CVB- D has protective effect on cerebral ischemia-reperfusion injury in rats.