微波诱变选育耐热糖化酶菌株

Breeding of Thermostable Glucoamylase Strains by Microwave Mutation

采用微波诱变糖化酶生产菌株Aspgillus niger 3.4309,用SDS双层梯度平板进行筛选,最终得到比出发菌株糖化酶耐热性提高15～20℃的突变株9-1,经多次传代证明该菌株遗传性能稳定。

Through microwave mutation of the strain Aspergillus niger 3.4309 （which could produce thermostable glucoamylase） and the screening of the mutants by double-layer plate containing SDS, the mutant strain 9-1 was eventually obtained, the thermostable performance of which increased by 15 -20 ℃ higher than that of the primitive strain. The passage of such strain for multiple generations also proved its stable heredity.