摘要
采用微波诱变糖化酶生产菌株Aspgillus niger 3.4309,用SDS双层梯度平板进行筛选,最终得到比出发菌株糖化酶耐热性提高15~20℃的突变株9-1,经多次传代证明该菌株遗传性能稳定。
Through microwave mutation of the strain Aspergillus niger 3.4309 (which could produce thermostable glucoamylase) and the screening of the mutants by double-layer plate containing SDS, the mutant strain 9-1 was eventually obtained, the thermostable performance of which increased by 15 -20 ℃ higher than that of the primitive strain. The passage of such strain for multiple generations also proved its stable heredity.
出处
《酿酒科技》
北大核心
2007年第4期37-39,共3页
Liquor-Making Science & Technology
基金
河北省生物工程重点学科资助
关键词
微生物
微波诱变
糖化酶
耐热性
microbe
microwave mutation
glucoamylase
thermostable