非洲菊组织培养抑制褐变现象的研究

Study on Inhibiting Browning Reaction of Gerbera jamesonii Bolus in vitro

为控制非洲菊组织培养中的褐变提供有效的解决方法,研究了不同外植体及抗氧化剂对非洲菊褐变现象的影响。试验结果表明,非洲菊幼嫩叶片、花蕾、花托和花梗等不同外植体中叶片的褐变程度最重,花蕾的褐变程度最轻。在非洲菊叶片组织培养时,接种前将外植体用100 mg/L Vc溶液浸泡1 h,再在以后的继代培养中加入200 mg/L PVP使褐变现象得到有效控制。

The different explants and antioxidants were tested to find the effective method of inhibiting browning reaction of gerbera in vitro. The results show that the browning degree of leaf was the most serious, and that of floweret was the lightest among young leaf, floweret, floral receptacle and floral peduncle, and soaking leaves 1 hour in 100 mg/L Vc before planting and adding 200 mg/L PVP during subculture could control browning reaction effectively.