摘要
目的：建立木通药材中木通苯乙醇苷B的HPLC分析方法。方法：采用Phenomenex Luna C18（4．6mm×250mm，5μm）柱，以甲醇．水．磷酸（35：65：0．05）为流动相，流速1．0mL·min^-1，检测波长330nm，柱温35℃。结果：木通苯乙醇苷B在0．04～0．86μg呈良好的线性关系，r=0．9998，平均回收率97．2％。对3个基源（三叶木通、五叶木通、白木通）的木通药材及其他部位（根、叶、果实）共39个样品进行了含量测定，各样品含量在0．021％～0．745％。结论：方法简单，重复性好，适合用于木通药材的质量控制。
Objective： To establish a quantitative method for calceolarioside B in Caulis Akebiae. Method： The samples were separated at 35℃ on a Phenomenex Luna C18 column eluted with methanol-water-phosphoric acid （35： 65： 0. 05） as mobile phase. Flow rate was at 1.0 mL · min ^-1 and the detection wavelength was at 330 nm. Result： The calibration curve was linear over the range from 0. 04 to 0. 860 g （r = 0. 999 8） and the average recovery was 97. 2%. 39 Crude drug materials collected from different areas were determined and the contents of calceolarioside B in Caulis Akebiae were fluctuated from 0. 021% to 0. 745%. Conclusion： The method is simple and repeatable and could be used for the quality control of Caulis Akebiae.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第6期476-478,共3页
China Journal of Chinese Materia Medica
基金
"863"创新药物和中药现代化项目(2004AA2237-3014)
