生地注射液对脂多糖诱导大鼠肺部炎症的影响

Shengdi injection on rat model of lung inflammation induced by lipopolysaccharides

目的:研究生地注射液对大鼠肺部炎症的影响。方法:气道滴入大肠杆菌脂多糖(LPS)建立大鼠肺部炎症模型;随机分正常对照组、模型组、药物组,各组均采用静脉注射给药方式,2次给药,模型组给生理盐水,药物组每次给生地注射液380mg.kg-1(以生地注射液中固形物量计),进行支气管肺泡灌洗液(BALF)中细胞分类计数;测定BALF中肿瘤坏死因子(TNF-α),超氧阴离子自由基(O2-),中性粒细胞髓过氧化酶(MPO)水平;进行肺组织病理检查。结果:生地注射液静脉给药可明显抑制LPS诱导的白细胞总数和中性粒细胞数目升高,对淋巴单核细胞数目无明显影响;明显抑制TNF-α,O2-,MPO水平升高;明显抑制LPS诱导的肺组织中性粒细胞浸润、气管黏膜水肿,对肺组织损伤无影响。结论:生地注射液有一定的抗LPS致大鼠肺部炎症的作用,初步推断抗炎、抑制细胞因子释放、抑制炎症介质释放和抗氧化是其治疗慢性阻塞性肺疾病的作用机制之一。

Objective： To investigate the effect of Shengdi injection on rat model of lung inflammation. Method： The rat model was established by intratrachea instillation of lipopolysaccharides （LPS）. The total and different white blood cell counts in bronchoalvoelar lavage fluid （BALF） were performed and the level of tumor necrosis factor-α （TNF-α）, superoxide anion radical （O^-2） and myeloperoxidase （MPO） was measured, as well as pathologic change of pulmonary tissue was tested. Result： Shengdi injection could depress the increasing of the amount of total white blood cells and neutrophils and inhibit the increasing of TNF-α, O^-2, MPO caused by LPS, as well as relieve the pathologic change including Neutrophils infiltrating and mucous edema in tracheae after intravenous administration. While it did not show the effect on monocyte, and histological lesion of the lung tissue. Conclusion： Shengdi injection shows some anti-inflammatory effect in rat lung induced by LPS and it can be concluded tentatively that anti-inflammatory, inhibiting the release of cytokine and inflammatory medium, and antioxidation are some of the mechanism of its effect on COPD.